乳胶微球产品

乳胶微球产品信息

Latex microsphere product information
产品描述：

Product Description:
乳胶微球在免疫诊断学方面有着非常广泛的应用。采用乳胶微球可以从血清、尿和脑脊液中检测出微量的抗原或者抗体。微量的抗体可以使抗原包被的微球产生明显的聚集；同样微量的抗原也可以使抗体包被的微球产生一样明显的聚集。

Latex microspheres have a very wide range of applications in immunodiagnosis.Latex microspheres can detect trace amounts of antigens or antibodies in serum,urine, and cerebrospinal fluid. Micro amounts of antibodies can causesignificant aggregation of antigen-coated microspheres; The same trace amountof antigen can also cause similar aggregation of antibody coated microspheres.

另外一种较常见的用途是将其应用在乳胶凝集抑制试验中。将待测样品中的抗原与已知抗体作用后，再与相应抗原包被的乳胶微球混合。因没有游离抗体的存在，乳胶颗粒表面的抗原不能与抗体结合出现凝集现象，即凝集被抑制。

Another common use is to apply it in latex agglutinationinhibition tests. After reacting the antigen in the test sample with knownantibodies, mix it with the corresponding antigen coated latex microspheres.Due to the absence of free antibodies, the antigen on the surface of latexparticles cannot bind to the antibody, resulting in agglutination, which issuppressed.

例如，孕妇尿液中含HCG，用抗HCG与之结合后，再加HCG包被的乳胶，则不出现凝集现象。乳胶微球也常常被应用于细胞吞噬研究，在此应用中常常使用粒径从小于50nm到3μm之间大小的微球。

For example, if the urine of pregnant women contains HCG, andanti HCG is combined with it, then latex coated with HCG is added, there willbe no agglutination phenomenon. Latex microspheres are often used in cellphagocytosis research, with particle sizes ranging from less than 50nm to 3 μMicrospheres of size between m.

现有羧基/氨基聚苯乙烯微球、羧基/氨基聚甲基丙烯酸甲酯微球和表面空白的聚苯乙烯微球三种微球。其中羧基/氨基聚苯乙烯微球和表面空白的聚苯乙烯微球的材质为聚苯乙烯高聚物，并用二乙烯基苯交联，形成网状聚合物结构；以及羧基/氨基聚甲基丙烯酸甲酯微球的材质为聚甲基丙烯酸甲酯。产品为水分散体系，有很好的力学性能、耐溶剂、耐各种pH环境，以及表面富含羧基/氨基和硫酸根，在溶液中可以保持很好的稳定性，可以适用于各种缓冲溶液。微球表面光滑、平整，比表面积大，并具有很好的均一性、单分散性和稳定性。此类羧基/氨基微球可以较容易地将羧基/氨基与蛋白进行共价偶联，而表面空白的聚苯乙烯微球可以与蛋白通过疏水缔合作用进行连接。在免疫比浊检查、免疫荧光检测、ELISA增强、乳胶凝集试验等领域有着广泛的应用。

There arethree kinds of microsphere: carboxyl/amino polystyrene microsphere,carboxyl/amino polymethyl methacrylate microsphere and polystyrene microspherewith blank surface. The carboxyl/amino polystyrene microspheres and the surfaceblank polystyrene microspheres are made of polystyrene high polymer andcrosslinked with divinylbenzene to form a network polymer structure; And thecarboxyl/amino polymethyl methacrylate microspheres are made of polymethylmethacrylate. The product is a water dispersible system with excellentmechanical properties, solvent resistance, and resistance to various pHenvironments. The surface is rich in carboxyl/amino groups and sulfateradicals, which can maintain good stability in the solution and can be appliedto various buffer solutions. The surface of the microspheres is smooth andflat, with a large specific surface area and good uniformity, monodispersity,and stability. These carboxyl/amino microspheres can easily covalently couplecarboxyl/amino groups with proteins, while surface blank polystyrenemicrospheres can be connected to proteins through hydrophobic association. Ithas wide applications in fields such as immunoturbidimetry, immunofluorescencedetection, ELISA enhancement, latex agglutination test, etc.

产品优势：
•       产品多样化：我们可以提供60nm~700nm之间各种粒径的微球；
•       良好的稳定性：常温放置可达24个月。
•       高度的均一性：PDI＜0.050；
•       表面羧基含量高：羧基含量在200μmol/g~1200μmol/g
•       独特的制备工艺：羧基牢固，不易脱落；
•       产品中无聚丙烯酸类含羧基的游离聚合物存在；
•       微球的强度和韧性佳：在应用中不易被机械破坏；

Product advantages:

Product diversification: We can provide microspheres withvarious particle sizes between 60nm and 700nm;

Good stability: can be stored at room temperature for up to 24months.

• Heightuniformity: PDI ＜ 0.050;

High surface carboxyl content: carboxyl content at 200 μMol/g~1200 μ Mol/g

Unique preparation process: The carboxyl group is firm and noteasy to peel off;

There are no free polymers containing carboxyl groups such aspolyacrylic acid in the product;

The strength and toughness of microspheres are excellent: theyare not easily mechanically damaged in applications;

产品用途

1).免疫比浊试剂，用作抗体、抗原载体.

2).作为酶制剂的普适性支撑材料，用作固定化酶载体

3).ELISA试剂盒开发，用作二抗与HRP载体

4).粒径标准品.

Product Usage

1) Immunoturbidimetric reagent, used as an antibody and antigencarrier

2) As a universal support material for enzyme preparation, as acarrier for Immobilized enzyme

3) Development of an ELISA kit as a carrier for secondaryantibodies and HRP

4) Particle size standard

应用领域

乳胶微球可以作为诊断试剂的原材料用于抗体／抗原分子的负载，在诊断试剂的应用中起到信号增强的作用。通过载体的放大作用，诊断试剂产品的检测灵敏度可以提高几个数岱级。主要可以应用在以下几方面

application area

Latex microspheres can be used as raw materials for diagnosticreagents to load antibody/antigen molecules, playing a role in signalenhancement in the application of diagnostic reagents. Through theamplification effect of the carrier, the detection sensitivity of diagnosticreagent products can be improved by several orders of magnitude. Mainlyapplicable in the following aspects

1免疫荧光检测

免疫荧光检测的原理是先将抗体固载于微球表面，负载于微球表面的抗体与抗原特异性结合后，再加入荧光标记的第二抗体与抗原结合，使微球－抗体－抗原－第二抗体－荧光标记复合物具有稳定的荧光信号，经过洗涤后，用仪器检测荧光信号。荧光信号的强度与披测抗原的浓度有较强的相关性。

目前市场上采用免疫荧光法的检测试剂产品有： (1)甲胎蛋白定撮检测试剂盒（免疫荧光法）： (2) 癌胚抗原定量测定试剂盒（时间分辨免疫荧光法）： (3) 乙型肝炎病毒表面抗原定岱测定试剂盒（时间分辨免疫荧光分析法）: (4) 支原体检测试剂盒（免疫荧光法）： (5) 抗核抗体 (ANA)间接免疫荧光法检测试剂盒：等等。

1. Immunofluorescence detection

The principle of immunofluorescence detection is to firstimmobilize antibodies on the surface of microspheres, and then add a secondantibody labeled with fluorescence to bind to the antigen. This results in astable fluorescence signal of the microsphere antibody antigen second antibodyfluorescence labeled complex. After washing, the fluorescence signal isdetected using an instrument. There is a strong correlation between theintensity of fluorescence signals and the concentration of detected antigens.

At present, immunofluorescence detection reagent products in themarket are: (1) AFP assay kit (immunofluorescence method); (2) Carcinoembryonicantigen quantitative assay kit (time-resolved immunofluorescence method); (3)hepatitis B virus surface antigen assay kit (time-resolved immunofluorescencemethod); (4) Mycoplasma detection kit (immunofluorescence method); (5)Antinuclear antibody (ANA) Indirect immunofluorescence assay kit, etc.

2胶乳颗粒增强免疫比浊法

胶孔颗粒增强免疫比浊法 (PETIA) 分为两种。一种是散射比浊检测法：另一种是透射比浊检测法。这两种方法的基本原理都是在高分子胶孔微球的表面交联抗体或抗原，当交联有抗体或抗原的微球与抗原或抗体结合后，在短时间内会迅速聚集在一起，改变了反应液的散光性能或透光性能。而且，反应液散光性能或透光性能（即吸光度）的改变与被测抗原或抗体的浓度有较强的相关性，在一定范围内可以反映被测物的浓度

目前市场上采用免疫比浊法的胶乳免疫比浊试剂产品有： (1) 脑肋肤检测试剂： (2) a1-微球蛋白检测试剂： (3)糖化血红蛋白检测试剂: (4)脂蛋白a检测试剂：

(5) 高敏C-反应蛋白检测试剂； (6) C-反应蛋白检测试剂： (7) 抗链球菌溶血素"O"检测试剂： (8)视黄醇结合蛋白检测试剂： (9)类风湿因子检测试剂：< 10) 132微球蛋白检洌试剂， (11) 中性粒细胞明胶酶相关脂质运载蛋白检测试剂： < 12) D-二聚体检测试剂： < 13) CK-MB 检测试剂: < 14) 胱抑素C 检测试剂： (15) 肌红蛋白检测试剂； (16) 甲胎蛋白检测试剂： (17) 肌钙蛋白检测试剂：(18) 心型脂肪酸结合蛋白检测试剂；等等。

2 latex particles enhanced immunoturbidimetry

There are two methods for enhancing immune turbidimetry usingcolloidal particles (PETIA). One is scattering turbidimetric detection, and theother is transmission turbidimetric detection. The basic principle of both methodsis to cross link antibodies or antigens on the surface of polymer gel porousmicrospheres. When the cross linked microspheres with antibodies or antigensare combined with antigens or antibodies, they will quickly gather together ina short time, changing the dispersion or transmittance of the reactionsolution. Moreover, there is a strong correlation between the changes in theastigmatism or transmittance (i.e. absorbance) of the reaction solution and theconcentration of the tested antigen or antibody, which can reflect theconcentration of the tested substance within a certain range

At present, latex immunoturbidimetric reagent products on themarket using immunoturbidimetry include: (1) brain rib skin detection reagent:(2) a1 microglobulin detection reagent: (3) Glycated hemoglobin detectionreagent: (4) lipoprotein a detection reagent:

(5) High sensitivity C-reactive protein detection reagent; (6)C-reactive protein test reagent: (7) anti Streptolysin "O" testreagent: (8) Retinol binding protein test reagent: (9) Rheumatoid factor testreagent:<10) 132 microglobulin test reagent, (11) neutrophil Gelatinaserelated lipid carrier protein test reagent:<12) D-dimer test reagent:<13)CK-MB test reagent:<14) Cystatin C test reagent: (15) Myoglobin testreagent; (16) Alpha fetoprotein test reagent: (17) Troponin test reagent: (18)heart type fatty acid binding protein test reagent; Wait a minute.

3 ELISA反应的增强

此类应用是基千ELISA反应的原理，使用粒径范围在 60-200nm的乳胶微球，用酶标仪进行检测。区别在于传统的ELISA反应采用了酶标抗体，而此应用是将酶和抗体一起偶联到微球的表面，使一个微球表面连接有多个酶分子和抗体分子，将偶联有大昼酶分子和抗体分子的微球代替传统的一个酶分子标记一个抗体的酶标抗体对ELISA反应起到一个增强的作用。

Enhanced ELISA response

This type of application is based on the principle of ELISAreaction, using latex microspheres with a particle size range of 60-200nm fordetection using an enzyme-linked immunosorbent assay (ELISA). The difference isthat traditional ELISA reactions use enzyme-linked antibodies, while thisapplication couples enzymes and antibodies to the surface of microspheres,connecting multiple enzyme molecules and antibody molecules on the surface of amicrosphere. Replacing the traditional enzyme-linked antibodies labeled withone enzyme molecule and one antibody, microspheres coupled with daytime enzymemolecules and antibody molecules enhance the ELISA reaction.

4乳胶凝集法

乳胶凝集法是以乳胶颗粒作为载体的一种间接；肚集试验。即吸附或偶联可溶性抗原或抗体千其表面，特异性抗体或抗原与之结合后，使该乳胶颗粒发生清晰凝集。凝集反应在玻瑶板上进行，结果在良好的光照下用肉眼观察，常用千类风湿因子检查等方面。

4 latex agglutination method

Latex agglutination method is an indirect method using latexparticles as carriers; Duji test. Namely, by adsorbing or coupling solubleantigens or antibodies onto their surfaces, specific antibodies or antigensbind to them, resulting in clear agglutination of the latex particles. Theagglutination reaction was carried out on a glass plate, and the results wereobserved with naked eyes under good light, and commonly used in thousands ofRheumatoid factor tests

羧基聚苯乙烯乳胶微球产品目录

以下为部分种类微球的马尔文激光粒度仪表征数据

Thefollowing are the characteristic data of some types of microspheres usingMarvin laser particle size instruments

同时我们对比了三种国外进口产品的马尔文激光粒度仪表征参数

At thesame time, we compared the characteristic parameters of Marvin laser particlesize instruments for three imported products from abroad